地塞米松通过抑制PPARγ表达促进滋养层细胞迁移功能
examethasone improves trophoblasts migration by down-regulating the expression of PPARγ
目的：探讨地塞米松是否能够通过调节PPARγ表达影响滋养层细胞的迁移功能。方法：培养人绒毛膜癌细胞株JEG-3，Real-time PCR检测地塞米松对PPARγ的mRNA表达调控，western blot检测地塞米松对PPARγ蛋白表达的影响。Transwell试验检测地塞米松和过表达PPARγ对滋养层细胞迁移功能的影响。结果：地塞米松可以使人滋养层细胞PPARγ表达下调。过表达PPAR-r可抑制滋养层迁移，地塞米松促进滋养层细胞迁移。结论：地塞米松可以下调PPARγ的表达，影响滋养层细胞的迁移功能，这也许可以解释PPARγ对胎盘发生的重要作用。
o explore whether dexamethasone can modulate trophoblast migration via regulating the expression of PPARγ. METHODS: The regulation of dexamethasone on PPARγ in cultured human choriocarcinoma cell line JEG-3 was analysed by real-time PCR and western blot.The Transwell analysis is taken for migration of dexamethasone or over-expression of PPARγ treated and control cells . Results: Dexamethasone inhibits PPARγexpression. PPARγoverexpression inhibits trophoblast migration while dexamethasone promote cell migration in JEG 3. Conclusion: Dexamethasone promotes the migration of trophoblast by down-regulating the expression of PPARγ, which may partly explain the important role of PPARγand dexamethasone in human placenta.
葛玉纯、段涛、阮晟鸣、蔡寒
基础医学妇产科学分子生物学
地塞米松过氧化物酶体增殖物激活受体γ迁移
dexamethasonePPARγmigration
葛玉纯,段涛,阮晟鸣,蔡寒.地塞米松通过抑制PPARγ表达促进滋养层细胞迁移功能[EB/OL].(2014-12-01)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201412-36.点此复制
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