甘蔗B12D基因cDNA全长克隆与表达分析

full length cDNA sequence of sugarcane gene was obtained from sugarcane(Saccharum officinarum ) stem full length cDNA library through large-scale sequencing and validated by the corresponding bioinformatics analysis, termed ScB12D (Genbank ：KF714497). The full length of ScB12D gene was 771 bp, with a 264 bp open reading frame (ORF), encoded 87 amino acids residues. The ScB12D of sugarcane was a basic protein, which has a conserved functional domains with the main function for energy metabolism, and this protein was located in plasma membrane. The mainly secondary structure element was random coil. Real-time quantitative PCR (RT-qPCR) analysis revealed that the expression of ScB12D in the NaCl stress was the highest in abiotic stresses and it showed slightly lower under the abscisic acid stress. Moreover, after inoculation with Sporisorium scitamineum, the expression of this gene decreased in the different time points. It can be to some extent inferred that the sugarcane B12D gene associated with the reaction mechanism of sugarcane to biotic and abiotic stresses. The results obtained in the present study will provide the basis of cloning and functional identification of other members of B12D in sugarcane and promote the use of B12D gene in sugarcane genetic engineering.