与小麦抗白粉病基因Pm6紧密连锁的STS标记

he powdery mildew resistance gene Pm6, which was from tetraploid wheat Triticum timopheevii L., is an effective gene in most powdery mildew epidemic areas of China. RFLP probe BCD135 has been proved linked with Pm6. In order to develop molecular markers for Pm6, in the present research, four STS primers (NAU/STSBCD135-1, NAU/STSBCD135-2, STS003 and STS004) were designed based on the sequence data of BCD135. These primers were used for PCR amplification using the genomic DNA of resistant near-isogenic lines (with Pm6) and their recurrent parent “Prins” as samples. No polymorphic product was observed when using primers STS003 and STS004. However, primers NAU/STSBCD135-1 and NAU/STSBCD135-2 could amplify two and one polymorphic bands between the near-isogenic-lines and “Prins”, respectively. The two primers were then used to amplify the F2 population from a cross of (IGV1-465 × Prins). The amplification and the powdery mildew resistance identification data were analyzed using the software Mapmaker3.0. The results indicated that both NAU/STSBCD135-1 and NAU/STSBCD135-2 were closely linked to Pm6 with a genetic distance of 1.0 cM.