法检测牛奶中的抗菌药物

In determination of antibacterial drug’s residues in milk, the result is often influenced by the number of indicator bacteria added into a milk sample when a TTC test is used. Therefore, a test for determination of the number of indicator bacteria was proposed before a TTC test was carried out. This study was designed to determine the minimum concentration of 9 common antibacterial drugs in bovine milk detectable by the TTC test. The results indicated that the limits for detection of penicillin, streptomycin, gentamicin, kanamycin, erythromycin, ciprofloxacin, oxytetracycline, aureomycin and sulfadiazine were 3μg/kg, 660μg/kg, 620μg/kg, 6200μg/kg,30μg/kg, 500μg/kg,100μg/kg,100μg/kg and 100μg/kg, respectively. The detectable limits for penicillin, erythromycin, oxytetracycline, aureomycin and sulfadiazine were equal to or bellow the Minimum Residue Limit Standards set by the Ministry of Agriculture of China. As the TTC test was simple and cheap with a broad spectrum of drugs detectable, it is suitable for the current status of dairy farms where various antibacterial drugs are used for disease control.