Grapevine leaf MALDI-MS imaging reveals the localisation of a putatively identified sucrose metabolite associated to Plasmopara viticola development
Grapevine leaf MALDI-MS imaging reveals the localisation of a putatively identified sucrose metabolite associated to Plasmopara viticola development
Abstract Despite well-established pathways and metabolites involved in grapevine-Plasmopara viticola interaction, information on the molecules involved in the first moments of pathogen contact with the leaf surface and their specific location is still missing. To understand and localise these molecules, we analysed grapevine leaf discs infected with P. viticola with MSI. Plant material preparation was optimised, and different matrices and solvents were tested. Our data shows that trichomes hamper matrix deposition and the ion signal. Results show that putatively identified sucrose presents a higher accumulation and a non-homogeneous distribution in the infected leaf discs in comparison with the controls. This accumulation was mainly on the veins, leading to the hypothesis that sucrose metabolism is being manipulated by the development structures of P. viticola. Up to our knowledge this is the first time that the localisation of a putatively identified sucrose metabolite was shown to be associated to P. viticola infection sites.
Eppe Gauthier、Silva Marta Sousa、Maia Marisa、Malherbe C¨|dric、Cordeiro Carlos、Cunha Jorge、McCann Andr¨|a、Figueiredo Andreia、De Pauw Edwin、Far Johann、Eiras-Dias Jos¨|、Quinton Lo?c
Mass Spectrometry Laboratory (MolSys), University of Li¨¨geLaborat¨?rio de FTICR e Espectrometria de Massa Estrutural, Faculdade de Ci¨oncias da Universidade de Lisboa||MARE - Marine and Environmental Sciences Centre, Faculdade de Ci¨oncias da Universidade de LisboaLaborat¨?rio de FTICR e Espectrometria de Massa Estrutural, Faculdade de Ci¨oncias da Universidade de Lisboa||Grapevine Pathogen Systems Lab (GPS Lab), Biosystems & Integrative Sciences Institute (BioISI), Faculdade de Ci¨oncias da Universidade de LisboaMass Spectrometry Laboratory (MolSys), University of Li¨¨geLaborat¨?rio de FTICR e Espectrometria de Massa Estrutural, Faculdade de Ci¨oncias da Universidade de Lisboa||MARE - Marine and Environmental Sciences Centre, Faculdade de Ci¨oncias da Universidade de LisboaEsta??o Vitivin¨acola Nacional, Instituto Nacional de Investiga??o Agr¨￠ria e Veterin¨￠ria (INIAV)Mass Spectrometry Laboratory (MolSys), University of Li¨¨geGrapevine Pathogen Systems Lab (GPS Lab), Biosystems & Integrative Sciences Institute (BioISI), Faculdade de Ci¨oncias da Universidade de LisboaMass Spectrometry Laboratory (MolSys), University of Li¨¨geMass Spectrometry Laboratory (MolSys), University of Li¨¨geEsta??o Vitivin¨acola Nacional, Instituto Nacional de Investiga??o Agr¨￠ria e Veterin¨￠ria (INIAV)Mass Spectrometry Laboratory (MolSys), University of Li¨¨ge
植物保护植物学生物化学
Vitis viniferametabolomicspathogen responsesucrose metabolism
Eppe Gauthier,Silva Marta Sousa,Maia Marisa,Malherbe C¨|dric,Cordeiro Carlos,Cunha Jorge,McCann Andr¨|a,Figueiredo Andreia,De Pauw Edwin,Far Johann,Eiras-Dias Jos¨|,Quinton Lo?c.Grapevine leaf MALDI-MS imaging reveals the localisation of a putatively identified sucrose metabolite associated to Plasmopara viticola development[EB/OL].(2025-03-28)[2025-05-18].https://www.biorxiv.org/content/10.1101/2022.08.01.502001.点此复制
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