拟南芥丝裂原活化蛋白激酶MPK3/MPK6参与调控ProPEPs基因的表达

Mechnical injury are challenging threats of plant survival. In Arabidopsis, plant elicitor peptides (Peps) are one kind of defense peptides produced in the wounded plant tissues. These peptides can enhance the plant defense responses. However, the regulatory mechanisms of these peptides remain unclear. In this paper, we revealed that mitogen-activated protein kinases MPK3/MPK6 are involved in the regulation of Peps precursors ProPEPs genes expression. In the conditional induced activation of MPK3/MPK6 system, the expression of ProPEPs genes are highly induced. The mutation of MPK3 or MPK6 reduces the expression of ProPEPs genes. WRKY33, a transcription factor downstream of MPK3/MPK6 is also involved in the expression of ProPEPs genes. Mutation of WRKY33 can compromise the induced expression of ProPEPs triggered by Botrytis cinerea. Furthermore, we found that Peps-induced ethylene production is independent of MPK3/MPK6 cascade.