Development of a chemiluminescence immunoassay to accurately detect African swine fever virus antibodies in serum
Development of a chemiluminescence immunoassay to accurately detect African swine fever virus antibodies in serum
Abstract The outbreak of African swine fever (ASF) has caused significant economic losses to animal husbandry worldwide. Currently, there is no effective vaccine or treatment available to control the disease, and therefore, efficient disease control is dependent on early detection and diagnosis of ASF virus (ASFV). In this study, a chemiluminescent immunoassay (CLIA) was developed using the ASFV protein p54 as a serum diagnostic antigen and an anti-p54 monoclonal antibody. After optimizing the working parameters of the CLIA, the sensitivity of the established CLIA was 1:128, ASFV-specific serum antibody was identified, and there was no cross-reaction with other swine virus antibodies. After testing 49 clinical serum samples, the consistency rate between the CLIA and the World Organization for Animal Health (OIE) recommended commercial kit was 100%. Thus, this CLIA had a high degree of specificity, sensitivity, and reliability, and could be used as a rapid detection method for epidemiological investigations of ASFV infection.
Yang Yong、Fan Junqing、Jiang Lili、Sun Xiaomei、Zhang Qiang、Jin Meilin、Zhao Ya、Lv Changjie
National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityNational Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityNational Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityNational Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityCollege of Biomedicine and Health, Huazhong Agricultural UniversityNational Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University||College of Veterinary Medicine, Huazhong Agricultural University||Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of AgricultureNational Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University
医学研究方法生物科学研究方法、生物科学研究技术
African swine fever virusp54 proteinmonoclonal antibodychemiluminescence immunoassay.
Yang Yong,Fan Junqing,Jiang Lili,Sun Xiaomei,Zhang Qiang,Jin Meilin,Zhao Ya,Lv Changjie.Development of a chemiluminescence immunoassay to accurately detect African swine fever virus antibodies in serum[EB/OL].(2025-03-28)[2025-04-30].https://www.biorxiv.org/content/10.1101/2021.02.28.433302.点此复制
评论