Unmodified, autologous adipose-derived regenerative cells improve cardiac function, structure and revascularization in a porcine model of chronic myocardial infarction
Unmodified, autologous adipose-derived regenerative cells improve cardiac function, structure and revascularization in a porcine model of chronic myocardial infarction
Abstract Numerous studies have investigated cell-based therapies for myocardial infarction (MI), with mixed results. In the present study the left anterior descending (LAD) artery of pigs was occluded for 180 min. Four weeks later, the mean left ventricular ejection fraction (LVEF) was shown to have been reduced to approximately 35%. At that time, 18×106 unmodified, autologous adipose-derived regenerative cells (UA-ADRCs) were delivered into the LAD vein (control: delivery of saline). Six weeks following UA-ADRCs/saline delivery, the mean LVEF had increased by 18% (p<0.01) after delivery of UA-ADRCs, but was unchanged after delivery of saline. This is among the best outcome ever reported in studies on porcine animal models of cell-based therapies for MI in which functional outcome was assessed with cardiac magnetic resonance imaging. The unique combination of the procedure used for isolating UA-ADRCs, the late cell delivery time and the uncommon cell delivery route applied in the present study may open new horizons for cell-based therapies for MI.
Schulz Daryl、Azares Alon、Alt Eckhard、Ghosn Mohamad、Karimi Tahereh、Haenel Alexander、Vykoukal Jody、Shah Dipan、Raizner Albert、Dave Amish、Kettlun Claudia、Valderrabano Miguel
The Methodist Hospital Research InstituteFlow Cytometry Core, Texas Heart Institute at St Luke?ˉs Episcopal HospitalHeart and Vascular Institute, Department of Medicine, Tulane University Health Science Center||The Methodist Hospital Research Institute||InGeneron, Inc.||Isar Klinikum MunichHouston Methodist DeBakey Heart and Vascular CenterHeart and Vascular Institute, Department of Medicine, Tulane University Health Science CenterHeart and Vascular Institute, Department of Medicine, Tulane University Health Science Center||The Methodist Hospital Research InstituteDepartment of Translational Molecular Pathology, MD Anderson Cancer Center, The University of TexasHouston Methodist DeBakey Heart and Vascular CenterHouston Methodist DeBakey Heart and Vascular CenterThe Methodist Hospital Research InstituteHeart and Vascular Institute, Department of Medicine, Tulane University Health Science CenterHouston Methodist DeBakey Heart and Vascular Center
医学研究方法基础医学临床医学
adipose-derived regenerative cellsstem cellsheart failuremyocardial infarctiontranslational medicine
Schulz Daryl,Azares Alon,Alt Eckhard,Ghosn Mohamad,Karimi Tahereh,Haenel Alexander,Vykoukal Jody,Shah Dipan,Raizner Albert,Dave Amish,Kettlun Claudia,Valderrabano Miguel.Unmodified, autologous adipose-derived regenerative cells improve cardiac function, structure and revascularization in a porcine model of chronic myocardial infarction[EB/OL].(2025-03-28)[2025-05-02].https://www.biorxiv.org/content/10.1101/286468.点此复制
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