重组人胰高血糖素样肽-1( 7-36)对体外胰岛细胞分泌功能的影响1
Study of the effects of recombined human glucagon-like peptide-1(7-36) on insulin secretion
目的：通过测定重组人胰高血糖素样肽-1（recombined human glucagon like peptide, rhGLP-1）对体外胰岛细胞分泌功能的影响，研究重组人胰高血糖素样肽-1的降糖作用。方法：体外原代培养的胰岛细胞，上清液中加入不同剂量的rhGLP-1和对照药物，通过酶联免疫法检测rhGLP-1的促胰岛素分泌作用。结果：一定剂量的rhGLP-1可刺激胰岛细胞分泌胰岛素，培养上清液中胰岛素的浓度有所增加。结论： rhGLP-1能促进离体胰岛细胞的分泌功能。
im: To study and analysis the effects of recombined human glucagon like peptide-1(7-36) on the secretary ability of pancreatic cells. Methods: The effects of rhGLP-1 on the secretary ability of cultured pancreatic cells was measured. Add different dosages of rhGLP-1 into the cultured pancreatic cells, and then the effects of rhGLP-1 was measured. by the methods of ELISA. Result: An effective method for the culture of pancreatic cells was established; Certain dosage of rhGLP-1 could stimulate pancreatic cells to secrete insulin. Conclusion: RhGLP-1 can promote the secretion function of pancreatic βcell, which suggests that rhGLP-1 possess the property of anti-diabetes.
李端、翁鸿博
基础医学生物科学理论、生物科学方法药学
重组人胰高血糖素样肽-1(rhGLP-1)糖尿病胰岛细胞培养胰岛素
recombined human glucagon like peptiderhGLP-1insulin，diabetes
李端,翁鸿博.重组人胰高血糖素样肽-1( 7-36)对体外胰岛细胞分泌功能的影响1[EB/OL].(2007-12-28)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/200712-840.点此复制
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