小花棘豆化学成分预试及薄层色谱分析

For further investigating the chemical principals of Oxytropis glabra DC and providing theoretical bases to prevention and treatment of locoweed poisoning and developing the medicinal value. Phytochemistry composition preliminary systematic and special tests and TLC were applied to analyze the chemical principals. The results showed that the plant contained oils, phenols, tannin, alkaloids, flavonoids, terpenoids, Saponins organic acid, chinones, et al. but no cardiac glycoside, cyanophoric glycoside and aliphatic nitro compounds; 10 Kg materials were hot reflux extracted with industrial alcohol for four times and 1167.5 g extractum was obtained. After dissolving with distilled water, the extractum was extracted with petroleum ether, chloroform, ethyl acetate, n-butanol in turn, and four parts were obtained as 172 g, 12.6 g, 30.7 g, 180.5 g which indicated that the principals were distributed in petroleum ether and ethyl acetate fractions; it was showed that 9, 7, 6, 5 kinds of principals were located in the four fractions after analyzing by TLC and swainsonine was contained in n-butanol fraction when comparing with the sample. The silica gel plates best developed were analyzed to determine the contents with planar chromatography image quantitative software, the results showed that Rf1, Rf7, Rf5, Rf1 points were the maximum content points in the four fractions respectively.