Modulation of bacterial cell size and growth rate via activation of a cell envelope stress response
Modulation of bacterial cell size and growth rate via activation of a cell envelope stress response
Abstract Fluctuating conditions and diverse stresses are typical in natural environments. In response, cells mount complex responses across multiple scales, including adjusting their shape to withstand stress. In enterobacteria, the Rcs phosphorelay is activated by cell envelope damage and by changes to periplasmic dimensions and cell width. Here, we investigated the physiological and morphological consequences of Rcs activation in Escherichia coli in the absence of stresses, using an inducible version of RcsF that mislocalizes to the inner membrane, RcsFIM. Expression of RcsFIM immediately reduced cellular growth rate and the added length per cell cycle in a manner that was directly dependent on induction levels, but independent of Rcs-induced capsule production. At the same time, cells increased intracellular concentration of the cell division protein FtsZ, and decreased the distance between division rings in filamentous cells. Depletion of the Rcs negative regulator IgaA phenocopied RcsFIM induction, indicating that IgaA is essential due to growth inhibition in its absence. However, A22 treatment did not affect growth rate or FtsZ intracellular concentration, despite activating the Rcs system. These findings suggest that the effect of Rcs activation on FtsZ levels is mediated indirectly through growth-rate changes, and highlight feedbacks among the Rcs stress response, growth dynamics, and cell-size control.
Miguel Amanda、Shi Handuo、Maier Lisa、Typas Athanasios、Verheul Jolanda、Valen David Van、Blaauwen Tanneke den、Zietek Matylda、Huang Kerwyn Casey、Sueki Anna
Department of Bioengineering, Stanford UniversityDepartment of Bioengineering, Stanford University||Department of Microbiology and Immunology, Stanford University School of MedicineGenome Biology Unit, EMBL HeidelbergGenome Biology Unit, EMBL HeidelbergFaculty of Natural Sciences, Mathematics, and Computer Science, Swammerdam Institute for Life Sciences, University of AmsterdamDepartment of Bioengineering, Stanford University||Department of Biology, California Institute of TechnologyFaculty of Natural Sciences, Mathematics, and Computer Science, Swammerdam Institute for Life Sciences, University of AmsterdamGenome Biology Unit, EMBL HeidelbergDepartment of Bioengineering, Stanford University||Department of Microbiology and Immunology, Stanford University School of Medicine||Chan Zuckerberg BiohubGenome Biology Unit, EMBL Heidelberg||Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences
微生物学分子生物学细胞生物学
Rcs phosphorelaycell shapeFtsZIgaARcsFA22cell divisionmorphogenesisgrowth rate
Miguel Amanda,Shi Handuo,Maier Lisa,Typas Athanasios,Verheul Jolanda,Valen David Van,Blaauwen Tanneke den,Zietek Matylda,Huang Kerwyn Casey,Sueki Anna.Modulation of bacterial cell size and growth rate via activation of a cell envelope stress response[EB/OL].(2025-03-28)[2025-05-26].https://www.biorxiv.org/content/10.1101/2022.07.26.501648.点此复制
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