小麦体细胞杂种渐渗系II-12 LMW-GS基因的克隆、鉴定和潜在育种价值的分析
LMW-GS genes in an somatic introgression line II-12 between Common wheat /Agropyron elongatum and their potential value in wheat breeding
为了发掘优质小麦谷蛋白基因资源，采用基因组PCR的方法，从小麦与长穗偃麦草体细胞杂种株系II-12中克隆得到了六个LMW-GS基因（1-50，2-11，2-34，1-31，1-36，1-95）。对这些基因序列进行分析，发现与以往克隆的LMW-GS基因具有高度的同源性。通过对推导氨基酸序列分析表明， 1-50，2-11，2-34可以产生成熟的多肽，因而可能是真基因；1-31，1-36，1-95由于存在提前终止信号可能是假基因。进一步的序列分析表明，1-50存在三个Cys残基参与分子间二硫键的形成；在2-34中,Gln 残基含量为34.29%，明显高于小麦LMW-GS中Gln残基平均水平。因而1-50和2-34可能与II-12的面粉优质相关的谷蛋白亚基。
In order to study the usefulness of low-molecular-weight glutenin subunits (LMW-GS) to wheat quality improvement, we characterized six LMW-GS genes, which were titled 1-50，2-11，2-34，1-31，1-36，1-95, from an introgression line II-12 between Triticum aestivum cv. Jinan177 (JN177) and Agropyron elongatum using genomic PCR method. All six sequences have high similar to those of wheat and related grasses reported before. Analysis of their deduced amino acid sequences suggests that 1-50，2-11，2-34 may be real genes and the other three are pseudogenes according to whether or not containing in-frame stop codon. Subunit 1-50 contains nine Cys residues and three may be involved in inter-molecular disulfide bonds; Glutamine in mature subunit 2-34, occupying about 34.29% of deduced amino acid, is significantly higher than the mean value of LMW-GS in common wheat. Thus, the two genes may be responsible for high quality of the hybrid line II-12.
赵峰、夏光敏、陈梦竹、陈凡国
农作物遗传学分子生物学
LMW-GS 体细胞杂种渐渗系 小麦 长穗偃麦草 面粉品质 基因组PCR
LMW-GS，somatic hybrid introgression line Triticum aestivum Agropyron elongatum flour quality genomic PCR
赵峰,夏光敏,陈梦竹,陈凡国.小麦体细胞杂种渐渗系II-12 LMW-GS基因的克隆、鉴定和潜在育种价值的分析[EB/OL].(2007-08-31)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/200708-477.点此复制
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