SARS-CoV-2 serological tests can generate false positive results for samples from patients with chronic inflammatory diseases
SARS-CoV-2 serological tests can generate false positive results for samples from patients with chronic inflammatory diseases
Abstract ObjectivesPatients with chronic inflammatory diseases are often treated with immunosuppressants and therefore are of particular concern during the SARS-CoV-2 pandemic. Serological tests will improve our understanding of the infection and immunity in this population, unless the tests give false positive results. The aim of this study was to evaluate the specificity of SARS-Cov-2 serological assays with samples from patients with chronic inflammatory diseases collected before April 2019, thus defined as negative. MethodsSamples from patients with multiple sclerosis (MS, n=10), rheumatoid arthritis (RA, n=47) with or without rheumatoid factor (RF) and/or anti-cyclic citrullinated peptide antibodies (anti-CCP2) and RF +/- systemic lupus erythematosus (SLE, n=10), were tested with 17 commercially available lateral flow assays (LFA), two ELISA kits and one in-house developed multiplex bead-based assay. ResultsSix LFA and the in-house IgG assay gave the correct negative results for all samples. However, the majority of assays (n=13), gave false positive signal with samples from patients with RA and SLE. This was most notable in RF positive RA samples. MS samples did not give any false positive in any of the assays. ConclusionThe majority of the verified serological assays were sensitive to interfering antibodies in samples from patients with chronic inflammatory diseases and therefore may have poor specificity in this context. For these patients, the risk of false positivity should be considered when interpreting results of the SARS-CoV-2 serological assays.
Kharlamova Nastya、Jerling Svante、Faustini Francesca、Gjertsson Inger、Pin Elisa、Nilsson Peter、Hober Sophia、Fink Katharina、Fogdell-Hahn Anna、R?nnelid Johan、Pullerits Rille、M?nberg Anna、Gunnarsson Iva、Dunn Nicky、Almgren Malin、Lundberg Karin、Bedri Sahl K
Department of Clinical Neuroscience, Karolinska Institutet||Center for Molecular MedicineDepartment of Clinical Neuroscience, Karolinska Institutet||Center for Molecular MedicineDepartment of Medicine Solna, Division of Rheumatology, Karolinska Institutet and Rheumatology, Karolinska University HospitalDepartment of Rheumatology and Inflammation Research, Institution of Medicine, Sahlgrenska Academy at University of GothenburgDivision of Affinity Proteomics, Department of Protein Science, KTH Royal Institute of TechnologyDivision of Affinity Proteomics, Department of Protein Science, KTH Royal Institute of TechnologyDepartment of Protein Science, KTH Royal Institute of TechnologyDepartment of Clinical Neuroscience, Karolinska Institutet||Department of Neurology, Karolinska University Hospital||Centrum for Neurology, Academical Specialist CentrumDepartment of Clinical Neuroscience, Karolinska Institutet||Center for Molecular MedicineDepartment of Immunology, Genetics and Pathology, Uppsala UniversityDepartment of Rheumatology and Inflammation Research, Institution of Medicine, Sahlgrenska Academy at University of Gothenburg||Department of Clinical Immunology and Transfusion Medicine, Sahlgrenska University HospitalDivision of Affinity Proteomics, Department of Protein Science, KTH Royal Institute of TechnologyDepartment of Medicine Solna, Division of Rheumatology, Karolinska Institutet and Rheumatology, Karolinska University HospitalDepartment of Clinical Neuroscience, Karolinska Institutet||Center for Molecular MedicineDepartment of Clinical Neuroscience, Karolinska Institutet||Center for Molecular MedicineCenter for Molecular Medicine||Department of Medicine Solna, Division of Rheumatology, Karolinska Institutet and Rheumatology, Karolinska University HospitalDepartment of Clinical Neuroscience, Karolinska Institutet||Center for Molecular Medicine
医学研究方法基础医学内科学
COVID-19AutoimmunityAutoantibodiesDiagnosticsRheumatoid Arthritis. Systemic Lupus ErythematosusMultiple SclerosisRheumatoid Factor
Kharlamova Nastya,Jerling Svante,Faustini Francesca,Gjertsson Inger,Pin Elisa,Nilsson Peter,Hober Sophia,Fink Katharina,Fogdell-Hahn Anna,R?nnelid Johan,Pullerits Rille,M?nberg Anna,Gunnarsson Iva,Dunn Nicky,Almgren Malin,Lundberg Karin,Bedri Sahl K.SARS-CoV-2 serological tests can generate false positive results for samples from patients with chronic inflammatory diseases[EB/OL].(2025-03-28)[2025-04-27].https://www.medrxiv.org/content/10.1101/2020.11.13.20231076.点此复制
评论