亚油酸异构酶在高山被孢霉中的异源表达
Heterologous expression of linoleic acid isomerase in oleaginous fungus Mortierella alpina
共轭亚油酸是一种由亚油酸异构酶催化合成的功能性脂肪酸。本文通过异源表达来自痤疮丙酸杆菌的亚油酸异构酶（PAI）基因实现了共轭亚油酸在PUFAs（Polyunsaturated fatty acids）工业生产菌株高山被孢霉中的生物合成。通过密码子优化及外源底物添加的方法使共轭亚油酸产量达到了高山被孢霉总脂肪酸含量的1.02%。本研究为用生物合成法生产共轭亚油酸开辟了新的途径，实现了高山被孢霉中外源性功能脂肪酸的生物合成。
onjugated linoleic acid (CLA) is a fatty acid that can be generated from linoleic acid (LA) by linoleic acid isomerase. In this study, the linoleic acid isomerase from Propionibacterium acnes was expressed heterologously, and the CLA was synthesized in Mortierella alpina, which is a polyunsaturated fatty acid (PUFA) industrial production fungi. The CLA content reached up to 1.02 % (w/w) of the total fatty acid in M. alpina via codon optimization of PAI gene and the addition of substrate in fermentation medium. Our research provides a new valuable approach for the biosynthesis of CLA and accomplishes the biosynthesis of exogenous functional fatty in M.alpina.
郝丹辉、陈永泉、张灏、陈海琴、陈卫
生物工程学生物化学微生物学
亚油酸异构酶高山被孢霉密码子优化异源表达
linoleic acid isomeraseMortierella alpinacodon optimizationheterologous expression
郝丹辉,陈永泉,张灏,陈海琴,陈卫.亚油酸异构酶在高山被孢霉中的异源表达[EB/OL].(2015-03-24)[2025-08-23].http://www.paper.edu.cn/releasepaper/content/201503-297.点此复制
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