活性炭层析柱分离PCB10和OCP16实验条件的优化

method for optimization on experimental conditions for separation of organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) by chromatographic column of activated carbon had been established. The condition of separating for sixteen of OCPs and ten kinds of PCBs were optimized by orthogonal experimental design of four factors three-level for activation temperature and activation time and granularity of activated carbon, types of eluting reagents. The results were showed that the optimization of separating between sixteen of OCPs and ten kinds of PCBs adsorbed in activated carbon with eluting of 30mL acetone and 10mL mix solution of acetone and dichloromethane and (95:5,v/v) in turn could be obtained in the conditions of activated carbon from 30 mesh to 80 mesh heating at 350℃ for an hour. The factors of main influence turn and turn about the activation temperature, the types of eluting reagents, the activation time and the granularity of activated carbon. It is feasible for this method to be suitable for eluting of the fractions in the column of activated carbon before determination of OCPs and PCBs in the sample of marine sediments by capillary gas chromatography with electric capture detector (GC-ECD).