新型荧光蛋白真核表达载体的构建及其瞬时表达

Fluorescent proteins are used intensively in cytology, medicine biology and molecular fields as they remain the property of fluorescence excitable activity after fused with other proteins fluently. However, with the advancement of fluorecent proteins study techonlogies, it demands more fluorescent variants’ occurrence. Neverthless, fluorecent proteins obtained traditionally restricted in extracting them directly from orgnism or site-directed mutagenesis and random mutation methods and little attentions payed on gene synthesis technology to get the novel fluorescent proteins. Here, we constructed the eukaryotic plasmid of pcDNA3.1(+)-G2-HexaHis based on the novel fluorescent proteins which were synthesized by Microfluidic PicoArray method. The constructed eukaryotic plasmid was then transferred into HeLa cells and the result showed that it can express considerably in the HeLa cells with intensive fluorescence. Besides, the subcelullar location result presented G2 mainly located in plasma and scarcely in nucleolus, so the fluorescent protein of G2 can be used in localization of proteins in plasma, marking cytoskeleton, cell mitosis and plasma protein expression intensity, meantime, it can also be used in marking specific protein of nucleus and proteins transferring between plasma and nucleus.