三七总皂苷对脑出血大鼠脑血肿周围组织PAR-1、NMDA-NR表达的影响

Objective:To investigate the effect of Panax Notoginseng Saponins(PNS) on the expression of PAR-1 and NMDA-NR of intracerebral hemorrhage rats(ICH).Methods:90 SD male rats were randomly divided into shame operation group,model group and treatment group,and time points of 6h，24h，48h，72h and 7d were observed.The shame operation group and model group were injected saline intraperitoneally,the treatment group was injected PNS intraperitoneally once a day.Both the expression of PAR-1 and NMDA-NR were measured by western blot meathod.Results:The expression of PAR-1 and NMDA-NR in the model group showed a trend of increase first at 6h time point,peaked at 48h,and then declined. The expression of PAR-1 and NMDA-NR reduced in the treatment group at each time point.There is sinifiacant difference of PAR-1 expression at each time point with the model group exceot at 6h point（P＜0.05）. There is sinifiacant difference of NMDA-NR expression at each time point with the model group exceot at 6h point and 7d point（P＜0.05）.Conclusion: The expression of PAR-1 and NMDA-NR of ICH rats increased. PNS may play a role in treating ICH by decreasing the expression of PAR-1 and NMDA-NR of ICH rats.