乳中β-酪蛋白含量微量测定方法的建立

In order to establish a way to quantitative determinate β-casein content in trace milk or biological specimen, capillary zone electrophoresis was used to detect β-casein content in mouse milk. In this research, β-casein in mouse milk was quantitative analyzed in 5 min by capillary zone electrophoresis. The running buffer was 20 mmol/L borax and boric acid buffer with pH 9.0. The running voltage was 20 kv and the detection wavelength was 200 nm. The results showed that β-casein content was highest in early lactation, then decreased and kept stable during the whole lactation period. All these results revealed that capillary zone electrophoresis was simple, rapid and accurate. It could be used for quantitative analyzing β-casein content in trace specimen.