丹参SmCPS1启动子区多态性与丹参酮类成分相关性研究

o investigate the relationship between SmCPS1 promoter polymorphism and the contents of tanshinones. We sequenced the SmCPS1 gene promoter region together with 388bp downstream from the translation start site and the content of tanshinones of 12 different germplasm of Salvia miltiorrhiza. To separate the functional polymorphic loci that may be related with the content of tanshinones from those not, the cis-elements of SmCPS1 promoter were predicted and analyzed by the Plant Transcriptional Regulatory Map database. We found 1) there was a different correlation between the polymorphism in the promoter region and the contents of the tanshinones. 2) Polymorphism on nine cis-elements, an 33bp insertion/deletion sequence and three tandem repeat variations in the sequence of SmCPS1 promoter may be related to the contents of tanshinones. These findings showed the relationships between SmCPS1 promoter polymorphism and tanshinones.