马铃薯Y病毒功能基因的扩增、克隆及生物信息学分析

【Object】In this paper, we have studied the eight important genes in potato virus Y with gene amplification and bioinformatic analysis. 。【Method】First, we extract RNA from potato leaves exhibiting typical symptoms and clone single gene, then translate these gene sequences into amino acid sequences，the last we import these sequences into related bioinfomatic software to gain the physicochemical properties and the parameters of the structure domains and the subcellular localization. We found three stable proteins (HC-Pro, CI and NIa) and five instable proteins (P1, P3, VPg, NIb and CP). According to the GRAVY, we can know these proteins can dissolved in water. We found a transmembrane in the latter half of P3 protein according to the TMHMM, at the same time we found no any signal peptide in the eight proteins according to the analysis of SignalP, hence there are no secretory proteins in these functional proteins. P1, HC, P3, CI, VPg and Nib have coil regions on the basis of the COILS Server analysis. The subcellular location by PSORT II Prediction suggest these proteins have more than one locations.