转录因子ATF6调控下游基因CTH表达并影响肝细胞癌进展

Objective: Explore the regulatory effect of transcription factor ATF6 on Hepatocellular carcinoma（HCC）and explain the influence of ATF6 on development and metastasis of HCC cells, verify the regulatory effect of ATF6 on the expression of the CTH gene, examine the production of H2S by CTH in HCC cells. Methods: Transfect HCC cells with ATF6 overexpression or knockdown plasmids and detect the effect of ATF6 on HCC cells through transwell migration, invasion and clonogenic survival assays, Western Blot and qPCR experiments were conducted to verify that ATF6 regulate the expression of CTH, H2S production was detected by Methyl blue method in CTH overexpressed HCC cells. Results: The migration and invasion of ATF6-overexpressed HCC cells increased significantly, ATF6 markedly increased the expression level of CTH mRNA and protein, H2S production in CTH-overexpressed HCC cells was significantly improved. Conclusion: ATF6 promotes the invasion, migration and growth of HCC cells, and improves the expression level of downstream gene CTH, CTH promotes the production of H2S in HCC cells, and ATF6 is involved in regulating the expression of CTH and affecting the progress of hepatocellular carcinoma.