34+细胞体外红系分化模型的建立及ZNF268的表达检测

Previous studies showed that ZNF268 gene might be involved in the erythroid hematopoietic development. To determine the expression of ZNF268 gene during erythropoiesis differentiation, CD34+ hematopoietic stem/progenitor cells be isolated and purified using immunomagnetic beads adsorption methods, and then be induced into erythropoiesis differentiation by combined-cytokines-induced methods. At different inducing stages, the cell surface molecular marks and changes in cell morphology be checked by flow cytometry and Wright's Giemsa staining, and ZNF268 expression in this process be detected. The results show that the cytokines-inducted erythroid differentiation of CD34+ cells in vitro be successfully developed and the the expression of ZNF268a and b2 encoded by ZNF268 gene decreased significantly during erythroid differentiation, and the results suggest that ZNF268 maybe play a role during the erythroid hematopoietic development.