Campylobacter jejuni modulates reactive oxygen species production and NADPH oxidase 1 expression in human intestinal epithelial cells
Campylobacter jejuni modulates reactive oxygen species production and NADPH oxidase 1 expression in human intestinal epithelial cells
Abstract Campylobacter jejuni is the major bacterial cause of foodborne gastroenteritis worldwide. Mechanistically, how this pathogen interacts with intrinsic defence machinery of human intestinal epithelial cells (IECs) remains elusive. To address this, we investigated how C. jejuni counteracts the intracellular and extracellular reactive oxygen species (ROS) in IECs. Our work shows that C. jejuni differentially regulates intracellular and extracellular ROS production in human T84 and Caco-2 cells. C. jejuni downregulates the transcription and translation of Nicotinamide adenine dinucleotide phosphate (NAPDH) oxidase (Nox1), a key ROS-generating enzyme in IECs and antioxidant defence genes cat and sod1. Furthermore, inhibition of Nox1 by diphenylene iodonium (DPI) and siRNA reduced C. jejuni ability to interact, invade and intracellularly survive within T84 and Caco-2 cells. Collectively, these findings provide mechanistic insight into how C. jejuni modulates the IEC defence machinery.
Wren Brendan、Corcionivoshi Nicolae、Gundogdu Ozan、Dorrell Nick、Elmi Abdi、Grabowska Anna D.、Omole Zahra、Liaw Janie、Davies Cadi、Hong Geunhye、Canonico Barbara
Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical MedicineBacteriology Branch, Veterinary Sciences Division, Agri-Food and Biosciences Institute (AFBI)Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical MedicineFaculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical MedicineFaculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical MedicineDepartment of Biophysics and Human Physiology, Medical University of WarsawFaculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical MedicineFaculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical MedicineFaculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical MedicineFaculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical MedicineDepartment of Biomolecular Sciences, University of Urbino Carlo Bo
基础医学微生物学分子生物学
Campylobacter jejuniIntestinal epithelial cellsNADPH oxidase 1Reactive oxygen species
Wren Brendan,Corcionivoshi Nicolae,Gundogdu Ozan,Dorrell Nick,Elmi Abdi,Grabowska Anna D.,Omole Zahra,Liaw Janie,Davies Cadi,Hong Geunhye,Canonico Barbara.Campylobacter jejuni modulates reactive oxygen species production and NADPH oxidase 1 expression in human intestinal epithelial cells[EB/OL].(2025-03-28)[2025-05-25].https://www.biorxiv.org/content/10.1101/2022.03.04.482506.点此复制
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