GF-β1对大鼠肝卵圆细胞上皮-间质转化的影响
Effect of TGF-β1 on epithelial-mesenchymal transition in rat hepatic oval cells
目的 检测转化生长因子β1（transforming growth factor, TGF-β1）能否在体外诱导大鼠肝卵圆细胞上皮-间质细胞转变（epithelial-mesenchymal transition，EMT）及其相应的信号转导机制。方法 在体外培养的大鼠肝卵圆细胞（WB F344）)中加入不同剂量的TGF-β1后，于48h后观察细胞形态的变化并收取细胞，分别采用Real-time PCR及Western blot检测上皮及间质细胞标志物表达的改变。结果 与TGF-β1未处理组相比，加入TGF-β1到WBF344细胞后，形态学上，TGF-β1诱导WB F344细胞向成肌纤维细胞样细胞转变,并且其间质细胞标志物表达上调，上皮细胞标志物的表达下调。随着TGF-β1剂量的加大，间质细胞标志物上调明显，上皮细胞标志物下调也明显（p<0.05）结论TGF-β1能在体外诱导大鼠肝卵圆细胞向间质细胞转变。
Purpose The aim of the present study was to investigate whether or not transforming growth factor-β1(TGF-β1)could induce epithelial-mesenchymal transition(EMT)of rat hepatic oval cells (WB F344)in vitro and its possible mechanism. Methods After the in vitro cultured WBF344 cells were treated with TGF-β1 at different doses．expression of the markers of epithelial cell and mesenchymal cell was assayed using Western blot and Real-time PCR analysis. Morphological alterations were examined by phase-contrast microscope. Results Incubation of WB F344 cells with TGF-β1 resulted in the up-regulation of the expression of the mesenchymocyte markers and the down regulation of the epithelial markers. With the increasing doses of TGF-β1, there were significantly higher expression of the mesenchymocyte markers and lower expression of the epithelial markers. Meanwhile，incubation of WBF344cells with TGF-βI could also lead to the transition of cells to myofibroblast-like cells morphologically．Conclusions TGF-β1 can induce EMT of WBF344 cells in vitro
饶慧瑛、刘峰、武楠、张薇、丛旭、魏来、费然
基础医学细胞生物学分子生物学
转化生长因子β1肝卵圆细胞上皮细胞-间质细胞转变大鼠
GF-β1hepatic oval cellepithelial - mesenchymal transition(EMT)rat
饶慧瑛,刘峰,武楠,张薇,丛旭,魏来,费然.GF-β1对大鼠肝卵圆细胞上皮-间质转化的影响[EB/OL].(2013-02-01)[2025-08-03].http://www.paper.edu.cn/releasepaper/content/201302-11.点此复制
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