sVR2和esVR2通过不同的机制调控食管癌细胞移动

Purpose: The aim of this study is to explore the roles and the underlying mechanisms of endogenous soluble vascular endothelial growth factor receptor-2 (esVR-2) and soluble vascular endothelial growth factor receptor-2 (sVR-2) in the migration of esophageal squamous cell carcinoma (ESCC) cells. Methods: ESCC cells were treated with different concentrations of recombinant sVR-2 protein and/or VEGF-C protein and the migrated capability of the cells was evaluated with Real-time cell detector (Real-Time Cell Analyzer (RTCA) Instruments). The effect of esVR-2 was further confirmed by gene forced-expression method. Results: Our results showed that: 1) Both recombinant VEGF-C protein treatment and VEGF-C overexpression could enhance the migration of ESCC cells; 2) Treatment of sVR-2 or overexpression of esVR-2 did not influence the migration of ESCC cells; 3) Co-transfection of esVR-2 and VEGF-C into ESCC cells could inhibit the VEGF-C-mediated promotion of cell migration; 4) When treated with both recombinant sVR-2 protein and VEGF-C protein (ratio: 5/1), the migration of ESCC was increaesd. Conclusions: sVR2 and esVR2 might play different roles in the migration of ESCC cells.