龙须藤叶粗提物的分离纯化及生物活性初探
龙须藤(Bauhinia championii Benth )具有抗炎镇痛、消除自由基、抗菌、抗肿瘤等功效。为进一步拓宽龙须藤的生物活性研究,探索其不同组分潜在的杀菌和除草活性,课题组通过常温冷浸提取法提取、真空浓缩得到甲醇提取物。采用硅胶柱层析分离纯化,经TLC检识和碘缸熏蒸后整合,得到9 个组分。反复重结晶7 号和8 号组分中析出的物质,经TLC检识和测熔点,得到1 个纯化合物,编号33 号,经波谱数据分析与molbase 库对照,鉴定该化合物为(1R, 2S, 3S, 4S, 5S, 6S) - 6 - 甲氧基- 1, 2, 3, 4, 5 - 环己烷五醇,是一种重要的工业原料。杀菌和除草活性试验结果显示,粗提物在1000 μg?mL-1 时对水稻稻瘟病菌的抑制率为40.84±1.00%,对稗草根的抑制率为49.18±2.33%;各组分在500 μg?mL-1 时,4 号组分对水稻稻瘟病菌的抑制率达到44.19±0.76%, 2 号和3 号组分对稗草根的抑制效果分别为88.92±1.31%和90.99±1.45%,3 号和6 号组分对马齿苋根的抑制效果分别为72.06±1.31%和89.92±1.73%。结果表明龙须藤叶提取物对水稻稻瘟病菌、稗草根和马齿苋根有良好的抑制效果,可进一步分离2 号、3 号、4 号、6 号组分,以期获得高活性的单体化合物。研究结果可为龙须藤作为新植物源农药的进一步研究与开发奠定基础。
Bauhinia championii Benth, a traditional Chinese herbal medicine, has effect on anti-inflammatory , analgesic, eliminate free radicals, anti-bacterial, anti-tumor and so on. In order to further broaden the potential fungicidal and herbicidal activity of different constituents of Bauhinia championii Benth, the research group attempt to dry and smash Bauhinia championii Benth leaves and use methanol to extract 3 times at normal temperature. Crude extract was isolated and purified on silica gel column chromatography. A bottle of fraction is collected every 200mL, according to the TLC detection and iodine cylinder integration, the crude extract was separated into 9 constituents.
姚宗理1,2;吴清来1,2;柯健1,2;黄冠1,2;杜晓英1,2;李俊凯1,2
植物保护农业科学研究药学
龙须藤,生物活性,甲醇提取物,分离纯化,单体化合物
姚宗理1,2;吴清来1,2;柯健1,2;黄冠1,2;杜晓英1,2;李俊凯1,2.龙须藤叶粗提物的分离纯化及生物活性初探[EB/OL].(2018-06-22)[2025-08-18].https://chinaxiv.org/abs/201806.00301.点此复制
评论