汽爆玉米秸秆产沼气及其古菌群落解析
Biogas production using steam exploded cornstalks and its archaea community composition
为研究汽爆玉米秸秆产沼气及其发酵过程中古菌群落变化，以汽爆玉米秸秆为原料在3% TS下进行批式中温发酵产沼气，检测发酵过程中pH、挥发性脂肪酸（VFAS）以及沼气产量的变化，同时取样利用梯度凝胶电泳（DGGE）法研究发酵过程中古菌群落的变化。结果表明：该系统启动迅速，料液在起始4天内产气量较大，但随着有机酸的累积造成的料液酸化，在中间段的产气量减小，料液总产气量达到9075mL。甲烷含量稳步上升，在第19天达到最高值，占总气体量的65.3%。DGGE分析表明系统中古菌多样性丰富，汽爆样品与对照样品间产甲烷菌种类多样性区别明显。甲烷粒菌属（Methanocorpusculum）是贯穿整个厌氧发酵过程的最主要的产甲烷菌。其中，在厌氧发酵产气前期，发酵体系中主要是以甲烷杆菌属（Methanobacterium）为主，而在厌氧发酵中后期则主要以甲烷球菌属（Methanococcus）和甲烷粒菌属（Methanocorpusculum）为主。汽爆玉米秸秆沼气系统古菌群落结构的阐明可为秸秆沼气工程调控提供科学数据。
With steam exploded cornstalks as raw material, a mesophilic batch fermentation study was carried out to investigate biogas production rate, pH and VFAS. And archaea composition was studied using denaturing gradient gel electrophoresis (DGGE). Fermentation started rapidly, and biogas prouduction rate was large within 4 days. However, the gas production decreased in the middle stage of fermentation because of the VFAS accumulating and the largest gas production could reach 9075 ml. Methane in the biogas increased stably and could be 65.3% of the total amount of gas. DGGE analysis showed that archaea diversity was rich. Archaea diversity of the steam exloded cornstalks treatment group was different with that of the control. Methanocorpusculum dominated in the whole fermentation period；Methanobacterium dominated in the early stage of fermentation period; Methanococcus and Methanocorpusculum dominated in the middle and later periods of the anaerobic fermentation. This research provides implications on the regulation of archaea composition for biogas production using steam exploded cornstalks.
李显耀、刘珂欣、刘丽英
生物工程学环境生物学微生物学
汽爆玉米秸秆沼气微生物群落GGE
steam exploded corn stalksbiogasArchaea communityDGGE
李显耀,刘珂欣,刘丽英.汽爆玉米秸秆产沼气及其古菌群落解析[EB/OL].(2017-06-02)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201706-40.点此复制
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