aATG8在小麦受叶锈菌侵染诱发的过敏性反应中的作用

Based on the wheat-leaf rust fungus interaction system, the roles of autophagy related gene TaATG8 during the hypersensitive reaction induced by fungal infection was discussed. RT-qPCR and western-blotting were applied to verify and present the mode of autophagy in this process. The results showed that the expression of gene in the RNA and protein level were significantly higher in the incompatible combination than those in the compatible combination at 36h after inoculation. This preliminarily indicated TaATG8 had participated the expression of defense response in the process of wheat resisting the fungus infection . After virus-induced gene silencing(VIGS) was used to silence TaATG8, hypersensitive reaction which induced by leaf rust infection began to expand, which indicated that autophagy may play a negative role in the hypersensitive reaction .