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野生茄子Na+/H+逆向转运蛋白基因(StNHX1)克隆与序列分析

lone and sequence analysis of a novel vacuolar Na+/H+ antiporter gene(StNHX1) from wild eggplant

中文摘要英文摘要

从野生茄子耐盐砧木品种'Torvum Vigor'叶片中克隆出液泡膜Na+/H+逆向转运蛋白耐盐基因(StNHX1;GeneBank登录号:JN606860.1),ORF长1,599 bp,共编码532个氨基酸。序列分析表明:StNHX1与番茄、马蔺、番杏、葡萄、盐穗木的NHX1同源性分别为93%、72%、72%、72%、71%。进化树分析表明,野生茄子StNHX1与番茄LeNHX1亲缘关系最近,其次是水稻OsNHX1,与海篷子SeNHX1亲缘关系最远。野生茄子'Torvum Vigor'液泡膜Na+/H+转运蛋白基因的克隆,为进一步开展增强植物耐盐能力的研究奠定基础。

novel vacuolar Na+/H+ antiporter gene from wild eggplant 'Torvum Vigor' (StNHX1; Accession No.: JN606860.1) was isolated. Sequence analysis showed that the ORF of StNHX1 gene is 1,599 bp, encoding 532 amino acids. Characteristic analysis indicated that StNHX1 shared high nucleotide sequences identity with the SlNHX1 from Solanum lycopersicum. The unrooted phylogenetic tree of StNHX1 and other species suggested that StNHX1 had the closest relationship with tomato and the furthest relationship with Salicornia europaea. Clone of the novel vacuolar Na+/H+ antiporter gene can lay the foundation of the research on plant salt tolerance ability.?????

杨立飞、陈国户、朱月林

分子生物学植物学

克隆Na+/H+逆向转运蛋白野生茄子序列分析StNHX1

loneNovel vacuolar Na+/H+ antiprterWild eggplantSequence analysisStNHX1

杨立飞,陈国户,朱月林.野生茄子Na+/H+逆向转运蛋白基因(StNHX1)克隆与序列分析[EB/OL].(2015-11-26)[2025-06-07].http://www.paper.edu.cn/releasepaper/content/201511-581.点此复制

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