赤芍水提物的药物血清对HSC-T6细胞促凋亡作用的实验研究

Objective To study the apoptosis-promoting effect of extract from Radix Paeoniae Rubra on hepatic stellate cell (HSC-T6)in the dog model.Methods the hepatic stellate cell (HSC-T6) were taken as the model in vitro established by using acetaldehyde. The Radix Paeoniae Rubra extract was given to dog orally once only. The serum samples were taken before giving Radix Paeoniae Rubra extract, 2 and 3 hours after giving Radix Paeoniae Rubra extract respectvely. The apoptosis-promoting effect of these serum samples(in 2% concentration) on HSC-T6 after 72 hours in the detected by flow cytometry..Results The serum sample promote obviously HSC-T6 apoptosis in 2 hour group and 3 hour group. The apoptosis rate was increased remarkably compare with that of the blank group （p<0.05）The proportion of HSC-T6 in G0/G1 phase were raised obviously in 2 hour group and 3 hour group（p<0.05）,while reduced in S phase（p<0.05）,There were no any changes in G2/M phase. Conclusion The serum samples of Radix Paeoniae Rubra extract all have obvious apoptosis -promoting effects in the two time points,raise the proportion of HSC-T6 in G0/G1 phase and reduce in S phase,which may be the mechanism of Radix Paeoniae Rubra extract for inhibitingv HSC-T6 proliferation of cells and promoting its apoptosis