肿瘤源exosomes诱导杀伤癌细胞的研究
Study on the killing and wounding activity of exosomes derived from tumour to carcinoma cell
目的 检测Tca－8113癌细胞来源的exosomes体外诱导刺激细胞毒性T淋巴细胞（cytotoxic T lymphocyte, CTL）杀伤肿瘤细胞的能力。方法 提取舌鳞癌Tca-8113细胞培养上清液中的exosomes，制备癌细胞的冻溶抗原(frozen tumor antigens，FTA)，并把两者分别负载到从血液分离和培养的树突状细胞上，测定其诱导的CTL对Tca-8113的杀伤活性, 以人肺腺癌SPCA-1细胞作对照。结果 体外FTA和exosomes冲击致敏的DC 能显著刺激T淋巴细胞增殖，其诱导的CTL对细胞系Tca-8113具有显著的杀伤作用，与IL－2培养的T细胞相比，差异具有显著性(P<0.01)；Exosomes冲击致敏的DC 诱导的CTL对人肺腺癌SPCA-1也有明显的杀伤作用(37.9±11.15%)，而FTA冲击致敏的DC 诱导的CTL无此功能。结论 肿瘤细胞培养上清液中分离出的exosomes，负载到DC上后，可以活化T细胞，使之成为抗原特异性的CTL，并具有特异性的杀伤肿瘤细胞的功能，为口腔癌的免疫治疗开拓了新的途径。Exosomes特异的CTL也可杀伤其他肿瘤细胞，说明肿瘤来源的exosomes是一种可引起肿瘤消退的共用抗原。
bjective To investigate the ability of exosomes derived from Tca-8113 to kill and wound cancer cells by inducing the production of tumor-specific T cells when pulsed onto dendritic cells. Methods Exosomes were extracted from Tca-8113 cell by ultrafiltration with Millipore centrifual Filter Devices; Frozen and thawed Tca-8113 cells to get frozen tumor antigens (FTA). The exosomes and FTA were pulsed onto DC generated from normal human peripheral blood mononuclear cell in vitro. The DC pulsed with FAP or exosomes were cocultured with the peripheral blood lymphocytes to transform T cell into specific CTL and observe the killing and wounding activity of CTL to cancer cells.SPCA-1cell was evaluated as control group. Results The CTL induced by DC pulsed with FTA or exosomes had significant activity to kill Tca-8113 (P<0.01);Moreover the CTL induced by DC pulsed with exosomes could also kill SPCA-1 cells(37.9±11.15%), but the CTL induced by DC pulsed with FTA had no this function. Conclusio
王霞、孙善珍、王东关、姜广水
肿瘤学基础医学细胞生物学
exosomes冻溶抗原ca－8113树突状细胞
ExosomesFTAca-8113dendritic cell
王霞,孙善珍,王东关,姜广水.肿瘤源exosomes诱导杀伤癌细胞的研究[EB/OL].(2005-06-13)[2025-04-26].http://www.paper.edu.cn/releasepaper/content/200506-36.点此复制
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