p73基因甲基化与非何杰金淋巴瘤的相关性研究

o investigate the effects of methylation of p73 on pathogenesis of non-Hodgkin lymphoma. The methylation status of p73 gene promoter and the expression of the p73 gene mRNA was examined in 24 NHLs by methylation-specific PCR (MSP) and reverse transcription polymerase chain reaction (RT-PCR) respectively; p73 protein was detected by Western blotting. Meanwhile, the expression of the p73 gene mRNA treated by 5-Aza-2'-deoxycytidine in several NHLs was analyzed. Methylation- specific PCR (MSP) reveals that the promoter of p73 gene was methylated in 21(87.5%) of 24 NHLs and not methylated in all of 12 reactive hyperplasia and 22 health adult peripheral blood.. p73 mRNA was not expressed in 20 (83.33%) of 24 NHLs and expressed in all of 12 reactive hyperplasia and 22 health adult peripheral blood, P73 protein was not expressed in 22 (91.67%) of 24 NHL and expressed in 12 reactive hyperplasia. p73 mRNA was expressed in several NHLs treated by 5-Aza-2'-deoxycytidine. p73 inactivation may be involved in the pathogenesis of NHLs and that methylation is the predominant mechanism of inactivation of p73 gene in NHL.