靶向DNAJB11基因的siRNA对卵巢癌细胞增殖、凋亡及侵袭的影响

Objective: Employing RNA interference technique to detect the relationship between DNAJB11 expression and cell proliferation, apoptosis and invasion of ovarian cancer. Methods: Chemical synthesis DNAJB11 siRNA and transferred to SKOV3 cell line; Using CCK-8 assay of detect cell proliferation; Using annexin V / PI staining flow cytometry to test cell apoptosis; Employing Transwell to test cell migration and invasion; Employing Western blot to detect DNAJB11, survivin and caspase-3 protein expression levels. Results: DNAJB11siRNA-290, DNAJB11siRNA-468, DNAJB11siRNA-1404 all decreased DNAJB11 gene expression, in which the effect of DNAJB11siRNA-468 was the most significant, with an inhibition rate over 80%. Compared with the control group, SKOV3 cell which deal with DNAJB11siRNA-468, cell proliferation decreased significantly with an inhibition rate over 50%. Flow cytometric analysis showed that the apoptotic rate of SKOV3 cells was significantly increased, 9.4% and 38.4% respectively, after transferred with DNAJB11siRNA-468 24h and 48h. Transwell experiment showed that after transfection of DNAJB11-siRNA468, the migration and invasion of SKOV3 cells significantly decreased. After DNAJB11 interference, survivin protein expression was significantly decreased and caspase-3 protein expression was significantly increased. Conclusion: DNAJB11siRNA inhibit cell proliferation, induce cell apoptosis and inhibit cell migration and invasion of SKOV3, by down-regulating survivin and caspase-3 expression. DNAJB11 was expected to be a new target therapeutic for patients with ovarian cancer.