基于FRET原理的新型硫化氢双光子荧光探针研究

s an important gasotransmitter, hydrogen sulfide (H2S) plays important role in regulating cardiovascular, neuronal and immune systems. It's concentration change is associated with various physiological processes and diseases. Although a lot of fluorescent probes for H2S have been reported, unfortunately, most of them have the fluorescence emission in the ultraviolet-visible (UV/Vis) spectral region, which renders them difficult to be employed in complicated biological samples. To address this address, we report a novel two-photon fluorescent probe for H2S based on fluorescence resonance energy transfer. This probe exhibited excellent sensitivity and selectivity under the complicated environment and can be used for detecting H2S in serum.