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锰胁迫对茶树锰含量和生理变化的影响

Effects of Mn stress on Mn content and physiological changes in tea plant

中文摘要英文摘要

为了研究锰对茶树生长生理的影响。通过检测不同浓度锰处理条件下(0.5、5、100、250和1000 uM)茶树叶片叶绿素含量、光合作用参数、叶绿荧光参数等分析锰对茶树品种'龙井长叶'的影响;根、茎和叶等不同组织中锰含量的差异、抗氧化系统中超氧化物歧化酶、过氧化物酶、多酚氧化酶的活性以及次生代谢产物咖啡碱、茶多酚和总氨基酸含量的差异。结果表明,随着锰浓度的升高,茶树的光合作用参数、叶绿荧光参数、叶绿素的含量、超氧化物歧化酶、过氧化物酶的活性呈现先升高后降低的变化趋势;茶多酚、咖啡碱含量减少;多酚氧化酶活性、总氨基酸含量增加。这表明低浓度锰(0.5和5 uM)对茶树生长具有促进作用,当锰浓度过高(大于 250 uM)对茶树生长起到抑制作用;研究还表明锰浓度对三种重要次生代谢产物的含量有较大影响。

To analyze the tolerance of tea plant(Camellia sinensis) to manganese (Mn) stress, concentrations of 5, 100, 250, and 1000 μM Mn were used to treat plants of 'Longjing changye' cultivar. Photosynthesis and chlorophyll fluorescence, as well as secondary metabolite contents, were investigated. The results show that the photosynthesis of C. sinensis was inhibited when Mn concentration was > 250 μM, and both antioxidation and plant protection systems were disrupted. We also discovered that the activities of superoxide dismutase (SOD) and peroxidase (POD) were decreased. As for accumulation of secondary metabolites after Mn stress treatment, total amino acid content increased while tea polyphenol content decreased, which can be attributed to stimulation of polyphenol oxidase (PPO) activity. However, Mn at low concentration (5 μM) promoted photosynthesis and C. sinensis growth. Presumably, C. sinensis growth inhibition under Mn stress was due to photosystem damage. Our results will provide a theoretical basis for further studies on C. sinensis tolerance to Mn and will be helpful for manganese fertilizing applications in various tea plantations.

甘可欣、朱旭君、李庆会、王晨赫

植物学生理学生物化学

茶学茶树生理变化

ea scienceTea plantMn stressPhysiological changes

甘可欣,朱旭君,李庆会,王晨赫.锰胁迫对茶树锰含量和生理变化的影响[EB/OL].(2017-04-28)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/201704-787.点此复制

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