神经干细胞移植促进脊髓损伤后轴浆运输通路重建的实验研究

Objective: To observe the effects of cryopreserved (-70℃) neural stem cells (NSCs) transplantation on the axon regeneration after the spinal cord injury(SCI) of rats. Methods: Neural stem cells were cultured from the hippocampus of the rat’s embryo and identified by immunocytochemistry of nestin, then NSCs were cryopreserved (-70℃) for 2 weeks. Seven days after the operation of SCI, we transplanted the NSCs into the injured site immediately. Sixty adult Wistar rats were randomly divided into three groups: spinal cord injury cured with transplants of NSCs to injuryed site (group A), spinal cord injury received DMEM solution (group B), control group(group C). Then NSCs labeled with Brdu were detected by immunohistochemisty, and the reconstruction of spinal cord were detected by horseradish peroxidase (HRP) staining. Result: NSCs can be detected in the spinal cord after transplantation. The number of HRP positive cells of group A is higher than group B. Conclusion: The transplantation of cryopreserved NSCs can survived in the injuried site and promote the reconstruction. It may be an progress of repairing the SCI by cells transplantation.