金复康的优化及其对肺腺癌细胞株A549增殖与凋亡的影响
the optimization of jinfukang and research about its effect on proliferation and apoptosis of A549 lung adenocarcinoma cancer cells
目的：对金复康全方进行优化，并观察金复康全方和金复康优化方醇提物对人肺腺癌A549细胞增殖和凋亡的影响。 方法：应用随机森林进行建模，对金复康12味药的全组合进行预测，最后得出62个方子，用5个不同浓度（62.5、125、250、500、1000 μg/ml）的62个组方醇提物分别作用于体外培养的A549细胞，48 h后采用CCK-8法检测62个组方对A549增殖的抑制作用，应用JMP软件分析出最佳的组合。通过克隆形成、细胞周期及凋亡检测，来验证金复康优化方确实要比全方对A549细胞的抑制效果更佳。结果：CCK-8结果表明7号方为最佳组方，应用JMP软件对62个组方CCK-8实验结果进行拟合分析，所得到的结果也是7号方最有效，这与CCK-8实验结果相一致，故7号方为金复康优化方。在抑制A549细胞增殖和克隆形成方面，金复康优化方都显著优于金复康全方（P<0.05）。流式细胞仪分析结果显示，金复康优化方在高浓度情况下将细胞周期阻滞于G2期，而金复康全方对A549细胞的周期无明显作用。与对照组相比，金复康全方和金复康优化方都能明显诱导A549细胞发生凋亡（P<0.05），且在高浓度组中，金复康优化方诱导细胞凋亡的效果明显优于金复康全方（P<0.05）。结论：金复康全方和金复康优化方对人肺腺癌A549细胞的增殖都有明显的抑制作用，且呈浓度依赖性。克隆形成、细胞周期和凋亡的实验结果一致显示在抑制A549细胞增殖，诱导细胞发生凋亡方面，金复康优化方的作用效果都显著优于金复康全方。
objective:optimize the jinfukang,and observe the effect of the alcohol extract of jinfukang and the optimization of jinfukang on the proliferation and apoptosis of human lung adenocarcinoma A549 cell. methods:use the random forest to build model, forecast the 12 herbs of jinfukang combination, finally obtain 62 prescription, using five different concentrations (62.5, 125, 250, 500 1000 ug/ml) of alcohol extract of 62 sets on the A549 cells, after 48h ,use cck8 to test the inhibition effect of 62 formula on A549 proliferation,JMP software is used to analyze the best combination.Verify that the optimization of jinfukang is better than jinfukang in terms of inhibiting A549 cell proliferation by detecting cell clone formation, cell cycle and cell apoptosis. Results:The result of CCK-8 showed that no. 7 is the best formula, using JMP software to fitting analyze the result of CCK-8 , and no. 7 is also the most effective, consistent with the result of CCK-8, no. 7 is the optimization of jinfukang. Jinfukang.In terms of inhibiting A549 cell proliferation, the optimization of jinfukang is better than jinfukang. In terms of inhibiting A549 cell clone formation,the optimization of jinfukang is better than jinfukang(P<0.05). flow cytometry showed that under the condition of high concentration,the optimization of jinfukang can block cell cycle in G2 phase, while jinfukang had no effect on cell cycle of A549.Compared with control group,the apoptosis rates of Jinfukang and the optimization of jinfukang induced increased significantly (P<0.05), and under the condition of high concentration, the apoptosis effect induced by the optimization of jinfukang is better than jinfukang(P<0.05) Conclusion: Jinfukang and the optimization of jinfukang both have inhibitory effect on proliferation of human lung adenocarcinoma A549 cells,present the concentration dependence, the result of cell clone formation、cell cycle and cell apoptosis showed that in terms of inhibiting A549 cell proliferation and inducing of cell apoptosis, the optimization of jinfukang is better than jinfukang.
焦丽静、阙祖俊、许玲、郑婷婷
肿瘤学药学中医学
金复康优化549细胞增殖凋亡
Jinfukangoptimization，A549，cell proliferation，apoptosis
焦丽静,阙祖俊,许玲,郑婷婷.金复康的优化及其对肺腺癌细胞株A549增殖与凋亡的影响[EB/OL].(2016-03-18)[2025-07-02].http://www.paper.edu.cn/releasepaper/content/201603-258.点此复制
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