|国家预印本平台
首页|草莓冷响应转录因子FaICE1克隆及其互作蛋白分析

草莓冷响应转录因子FaICE1克隆及其互作蛋白分析

loning of cold response transcription factor FaICE1 from strawberry and analysis of it's interacting proteins

中文摘要英文摘要

低温是影响冬季温室草莓生产的重要因素之一,克隆并鉴定抗冷相关基因对草莓理论研究和实际生产均有重要意义。本文利用八倍体草莓(Fragaria × ananassa)品种'红颜'作为试验材料,克隆并鉴定了一个低温响应的转录因子,FaICE1。基因表达分析结果显示,随着发育进程的推进,在果实发育前期FaICE1的表达呈现下降的趋势,白果期后其表达趋于稳定。同时,果实圆片试验表明低温胁迫(4℃)和茉莉酸(JA)均能够诱导FaICE1的表达。双分子荧光互补(BiFC)试验进一步证明,FaICE1与草莓果实抗低温关键信号蛋白FaSnRK2.6存在直接蛋白互作,暗示FaICE1可能是FaSnRK2.6的下游信号组分,参与了低温条件下草莓果实发育和成熟的调控。

Low temperature is one of the important factors affecting strawberry production in winter greenhouse. Cloning and identification of genes related to cold resistance have important significance for theoretical research and practical production of strawberry. In this paper, a transcription factor response to low temperature was cloned and identified in octoploid strawberry (Fragaria × ananassa). The expression of FaICE1 gene decreased at the early stage of fruit development, and became stable after the stage of white fruit. The experiment showed that the expression of FaICE1 was induced by low temperature(4 ℃) stress and jasmonic acid (JA), respectively. It was further proved that FaICE1 interacted directly with FaSnRK2.6, which is the key signal protein of strawberry fruit resistance to low temperature, it's suggesting that FaICE1 might be the downstream signal component of FaSnRK2.6 and participated in the development and ripening regulation of strawberry fruit under low temperature stress.

杜平、贾文锁、魏灵芝、李冰冰、李基正、贾美茹

园艺植物学生物科学研究方法、生物科学研究技术

草莓低温FaICE1FaSnRK2.6果实发育和成熟

strawberrylow temperatureFaICE1FaSnRK2.6fruit development and ripening

杜平,贾文锁,魏灵芝,李冰冰,李基正,贾美茹.草莓冷响应转录因子FaICE1克隆及其互作蛋白分析[EB/OL].(2016-11-01)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/201611-10.点此复制

评论