基于3D-rGO@Au的高灵敏电化学免疫传感器用于检测ProGRP
Ultrasensitive electrochemical immunosensor for ProGRP detection based on 3D-rGO@Au nanocomposite
为了提高对肺癌标记物ProGRP的检测灵敏度，制备一种基于三维碳纳米材料的夹心型电化学免疫传感器。通过水热法一步合成3D-rGO@Au作电极修饰材料，用于负载识别抗体。SiO2负载捕获抗体，用于特异性识别ProGRP并形成夹心结构。在最佳条件下，构建的传感器表现出较高的灵敏度，较宽的线性范围(1-10 ng/mL)和较低的检测限(0.136 fg/mL)。并且具有良好的时间稳定性、制备重现性和目标物选择性，对人血清中ProGRP也有良好分析性能。分析结果得出以下结论：3D-rGO@Au可以显著增大初始电流，而SiO2可以显著降低响应电流。这两种纳米材料的协同作用可以放大响应信号，提高传感器的灵敏度。
o improve the detection sensitivity of ProGRP, a sandwich electrochemical immunosensor based on three-dimensional carbon nanomaterials was prepared.3D-rGO@Au was synthesized by hydrothermal method in one step as electrode modification material for load recognition antibody.SiO2 loaded trapping antibody was used to specifically recognize ProGRP and form sandwich structure.Under the optimal conditions, the constructed sensor showed a high sensitivity, a wide linear range (1-10 ng/mL) and a low detection limit (0.136 fg/mL).Moreover, it has good stability, reproducibility and selectivity, and also has good performance for the analysis of ProGRP in human serum.The analysis results show that 3D-rGO@Au can significantly increase the initial current, while SiO2 can significantly reduce the response current.The combination of these two nanomaterials can amplify the response signal and improve the sensitivity of the sensor.
董盛谊、刘松、司思思
医学研究方法基础医学生物科学研究方法、生物科学研究技术
胃泌素释放肽前体电化学免疫传感器三明治结构三维纳米复合材料
pro-gastrin-releasing peptideelectrochemical immunosensorsandwich-type3D nanocomposite
董盛谊,刘松,司思思.基于3D-rGO@Au的高灵敏电化学免疫传感器用于检测ProGRP[EB/OL].(2021-05-14)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/202105-90.点此复制
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