家蚕中PGRMC2蛋白的转录、功能分析

he gene PGRMC2 is a novel gene from silkworm, Bombys mori. Up to now, there is no literature reporting about any characters about this gene in silkworm. We obtained the gene sequence which encoding PGRMC2 protein of Bombyx mori from the silkworm pupa cDNA library constructed by our lab with the GeneBank Accession Number was DQ311390. PGRMC2 contains an open reading predicted molecular weight of 26.05kD. The gene was inserted into a vector pET-28a with both EcoRⅠ and XhoⅠ restriction sites and then expressed in E. coli Rosseta (DE3). The target protein was purified by affinity chromatography and used to immune a male New Zealand rabbit for the production of anti-serum. Total protein of tissues of the fifth instar larva (Bombyx mori) were extracted and analyzed by Western blot. The results showed that PGRMC2 protein widely exist in the tissues of fifth instar larva (Bombyx mori). Meanwhile, the result of RT-PCR shows that there were high transcriptional level in ovary and testis, and higher with the development of silkworm. So we could deduce PGRMC2 played a very important role in the regulation of the growth and development of silkworm. This result laid a good foundation of further studies of PGRMC2.