丙酮酸盐对低血糖诱导的海马神经细胞死亡的影响

Objective To study the effects of pyruvate on hypoglycemia induced neuronal cell death of hippocampus. Methods The study was divided into control group，hypoglycemia group，and hypoglycemia + pyruvate group. Propidium iodide (PI) method was used to quantify neuronal death of hippocampus. Luciferin-luciferase luminescence assay was used to quantify ATP levels. Results Hypoglycemia group compared with control group showed that the neuronal cell death in CA1 of hippocampus increased significantly and the ATP levels of hippocampus decreased significantly (P<0.05). Pyruvate group compared with hypoglycemia group showed that the neuronal cell death in CA1 of hippocampus decreased significantly and the ATP levels of hippocampus increased significantly (P<0.05). Conclusions Pyruvate could ameliorate hypoglycemia induced the neuronal cell death; pyruvate could preserve the ATP levels to play a neuronal protection.