卫气月周期性变化与代谢标志物的相关性研究

Objective:To use metabonomics method to study the change of the basic material of month rhythm of Wei Qi, and to find the potential markers, as the essence of the Wei Qi theory research opens up a new way. Methods: Chinese lunar calendar in August the first day for early group, the 15th day for medium group, the 30th day for late group. The 10 rats were collected blood at the corresponding date of the 12:00 points.High performance liquid chromatography mass spectrometry instrument(HPLC-MS) was used to detect plasma metabolites, and principal component analysis(PCA)and partial least squares(PLS) was used to make statistical analysis to the data for comparing plasma metabonomics original data of each group,and explore the differences between groups in possible metabolic marker.Meanwhile coniecture potential biomakers of month rhythm of Wei Qi. Results: Compared with medium group, sphingosine-1-phosphate, phytosphingosine, cortisol, eicosapentaenoic acid, chenodeoxycholic acid sulfate, proline, dimethyl fumarate of early group as possible markers are to distinguish between the two groups; Linoleamide,sphingosine-1-phosphate, phosphatidyl glycerol, eicosapentaenoic acid, proline, cortisol, dimethyl fumarate, carnitine of late group as possible markers are to distinguish between the two groups. Compared with late group, cholesteryl acetate,proline, dimethyl fumarate, cortisol, phytosphingosine, carnitine, linoleamide, eicosapentaenoic acid, glycoursodeoxycholic acid of early group as possible markers are to distinguish between the two groups. Speculation eicosapentaenoic acid, cortisol, proline, dimethyl fumaratefor month rhythm potential markers. Conclusion: Month rhythm of Wei Qi may be related to lipid metabolism, amino acid metabolism closely related, and also accompanied by regulation of hormone.