蜘蛛消化液中抗菌活性分子的分离纯化

he main goal of the present study was to investigate the antimicrobial activity of the spider digestive juice in preliminary analysis and to isolate antimicrobial molecular. To begin with, we defined the spider digestive juice can kill microbe by the test of antimicrobial activity. Then the crude digestive juice were purified by centrifugal ultrafiltration and high performance liquid chromatography (HPLC).The eluted fractions were collected. Each fraction was further performed the test of antimicrobial activity.The fraction eluted at about 30 min is antimicrobial and nemed L. We tested the MIC to determine antimicrobial activity.