Fibroblast fusion to the muscle fiber regulates myotendinous junction formation
Fibroblast fusion to the muscle fiber regulates myotendinous junction formation
Summary Vertebrate muscles and tendons are derived from distinct embryonic origins yet they must interact in order to facilitate muscle contraction and body movements. How robust muscle tendon junctions (MTJs) form to be able to withstand contraction forces is still not understood. Using techniques at a single cell resolution we reexamined the classical view of distinct identities for the tissues composing the musculoskeletal system. We identified fibroblasts that have switched on a myogenic program and demonstrate these dual identity cells fuse into the developing muscle fibers along the MTJs facilitating the introduction of fibroblast-specific transcripts into the elongating myofibers. We suggest this mechanism resulting in a hybrid muscle fiber, primarily along the fiber tips, enables a smooth transition from muscle fiber characteristics towards tendon features essential for forming robust MTJs. We propose that dual characteristics of junctional cells could be a common mechanism for generating stable interactions between tissues throughout the musculoskeletal system.
Kraft-Sheleg Ortal、Zaffryar-Eilot Shelly、Hasson Peleg、Melamed Shay、Sun Chengyi、Yaseen-Badarneh Wesal、Millay Douglas P.
Department of Genetics and Developmental Biology, The Rappaport Faculty of Medicine and Research Institute, Technion ¨C Israel Institute of TechnologyDepartment of Genetics and Developmental Biology, The Rappaport Faculty of Medicine and Research Institute, Technion ¨C Israel Institute of TechnologyDepartment of Genetics and Developmental Biology, The Rappaport Faculty of Medicine and Research Institute, Technion ¨C Israel Institute of TechnologyDepartment of Genetics and Developmental Biology, The Rappaport Faculty of Medicine and Research Institute, Technion ¨C Israel Institute of TechnologyDivision of Molecular Cardiovascular Biology, Cincinnati Children?ˉs Hospital Medical CenterDepartment of Genetics and Developmental Biology, The Rappaport Faculty of Medicine and Research Institute, Technion ¨C Israel Institute of TechnologyDivision of Molecular Cardiovascular Biology, Cincinnati Children?ˉs Hospital Medical Center||Department of Pediatrics, University of Cincinnati College of Medicine
细胞生物学分子生物学生理学
muscle-tendon junctionmuscle fibertransdifferentiationfibroblastLoxL3
Kraft-Sheleg Ortal,Zaffryar-Eilot Shelly,Hasson Peleg,Melamed Shay,Sun Chengyi,Yaseen-Badarneh Wesal,Millay Douglas P..Fibroblast fusion to the muscle fiber regulates myotendinous junction formation[EB/OL].(2025-03-28)[2025-04-26].https://www.biorxiv.org/content/10.1101/2020.07.20.213199.点此复制
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