细胞外基质生物材料增强干细胞抗氧化应激诱导的早衰
ecellularized extracellular matrix enhances stem cells' resistance to oxidative stress-induced premature senescence
目的：研究细胞外基质生物材料对干细胞氧化应激性早衰的影响，增强早衰干细胞的增殖能力。方法：干细胞分别在普通塑料培养基材（TCPS）和脱细胞基质（DECM）上培养，加入适量的过氧化氢诱导干细胞发生早衰，检测β-半乳糖苷酶染色阳性细胞、细胞活性、增殖能力，采用免疫印迹法检测相关信号通道。结果：DECM生物材料能有效抑制β-半乳糖苷酶染色阳性细胞比率，增强干细胞的活性和增殖能力，并且DECM生物材料能显著降低干细胞胞内p38磷酸化程度和p16INK4a表达量。结论：干细胞在细胞外基质生物材料上培养时可有效抵抗氧化应激诱导的氧化损伤，预防早衰发生，保持MSCs自我更新和定向分化潜能。
Objective: To evaluate the effect of decellularized extracellular matrix (DECM) on oxidative stress-induced premature senescence in mesenchymal stem cells (MSCs). Methods:Sublethal dosages of hydrogen peroxide were used to induce senescence in MSCs.Senescence-associated β-galactosidase activity, cell viability, and cell proliferation were evaluated. To further reveal the underlying mechanisms, Western blot was used to analyze the intracellular signaling pathways. Results: Decellularized matrix enhanced the resistance to premature senescence, evidenced by the suppression of senescence-associated phenotypes, including decreased senescence-associated β-galactosidase activity, improved cell proliferation. The underlying molecular mechanisms involved the p38-p16 signaling pathway.Conclusions: These findings provide a new strategy of using stem cell-deposited matrix to overcome the challenge of cellular senescence and to facilitate the clinical application of MSCs in regenerative medicine.
陈曦、何帆、周龙
细胞生物学生物化学分子生物学
人工器官与生物医学材料学细胞外基质间充质干细胞氧化应激早衰
rtificial organ and biomedical materialsextracellular matrixmesenchymal stem cellsoxidative stresspremature senescence
陈曦,何帆,周龙.细胞外基质生物材料增强干细胞抗氧化应激诱导的早衰[EB/OL].(2016-05-13)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201605-329.点此复制
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