im-3分子表达异常与原因不明复发性流产的关系

Objective: To explore the relationship between abnormal expression of T cell immunoglobulin- and mucin-domain containing molecular-3 (Tim-3) and unexplained recurrent spontaneous abortion (URSA). Method: 31 pregnant women suffered with URSA were recruited as the study group and 30 normal pregnant women were chosen as the control group. For the two groups, CD4+Tim-3+cells/CD4+cell percentage in peripheral blood mononuclear (PBMC) were detected by flow cytometry. Then CD4+Tim-3+cells were collected by magnetic activated cell sorting. The expression levels of Tim-3 mRNA in PBMC, CD4+Tim-3+cells and decidua were detected by fluorescent quantitative RT-PCR. Besides, the relative amount of Tim-3 protein were also analyzed using Western blot. Result: In URSA group, the CD4+Tim-3+cells/CD4+cell percentage in PBMC was 5.23±0.96%, the relative level of Tim-3 protein in decidua was 0.64±0.09, the relative amounts of Tim-3 mRNA in PBMC and decidua were 0.65±0.09 and 0.50±0.07 respectively, which were all significantly higher that those in the control group. However, the expression of Tim-3 mRNA on Th1 cells in the URSA group was statistically lower than that in normal control. Conclusion: Down-regulation of Tim-3 on Th1 cells and excessive activation of Th1 might contribute to the development of URSA.