纺锤体检测点蛋白BUB1B在胃癌组织中的表达及意义

Objective To study the expression of sp indle checkpoint p rotein BUB1B and explore the significant value of BUB1B in the initiation of gastric cancer. Methods (1)The mRNA expression levels of BUB1B were compared in tumor tissues of 30 gastric cancer patients with 17 corresponding normal tissues by real-time quantitative PCR. (2) The Immunohistochemistry was used to evaluate of BUB1B protein expression. Result (1) BUB1B mRNA expression levels were higher in gastric cancer than in corresponding normal tissues, neary 8.875±1.08. (2) The result of Immunohistochemistry showed , BUB1B protein expression were higher in gastric cancer than in corresponding normal tissues, according to the standard scoring , the mean BUB1B protein expression of corresponding normal tissue was 92.12±12.99 and the mean expression of gastric cancer was 133.39±8.49 (p<0.05). And the expression of BUB1B is evidently related with TNM stage, but there was no evident correlation with lymph nodes metastasis, age, tumor size and family history. Conclusion The overexpression of BUB1B is related with the progression of gastric cancer and provides an new thread to explore the mechanism of cancer initiation and the effective treatments.