An evolutionarily ancient Fatty Acid Desaturase is required for the synthesis of hexadecatrienoic acid, which is the main source of the bioactive jasmonate in Marchantia polymorpha
An evolutionarily ancient Fatty Acid Desaturase is required for the synthesis of hexadecatrienoic acid, which is the main source of the bioactive jasmonate in Marchantia polymorpha
SUMMARY Jasmonates are fatty acid derived hormones that regulate multiple aspects of plant development, growth and stress responses. Bioactive jasmonates, defined as the ligands of the conserved COI1 receptor, differ between vascular plants and bryophytes (using jasmonoyl-L-isoleucine; JA-Ile and dinor-12-oxo-10,15(Z)-phytodienoic acid; dn-OPDA, respectively). Whilst the biosynthetic pathways of JA-Ile in the model vascular plant Arabidopsis thaliana have been elucidated, the details of dn-OPDA biosynthesis in bryophytes are still unclear. Here, we identify an ortholog of Arabidopsis Fatty Acid Desaturase 5 (AtFAD5) in the model liverwort Marchantia polymorpha and show that FAD5 function is ancient and conserved between species separated by more than 450 million years of independent evolution. Similar to AtFAD5, MpFAD5 is required for the synthesis of 7Z-hexadecenoic acid. Consequently, in Mpfad5 mutants the hexadecanoid pathway is blocked, dn-OPDA levels almost completely depleted and normal chloroplast development is impaired. Our results demonstrate that the main source of dn-OPDA in Marchantia is the hexadecanoid pathway and the contribution of the octadecanoid pathway, i.e. from OPDA, is minimal. Remarkably, despite extremely low levels of dn-OPDA, MpCOI1-mediated responses to wounding and insect feeding can still be activated in Mpfad5, suggesting that dn-OPDA is not the only bioactive jasmonate and COI1 ligand in Marchantia.
Jimenez-Alem¨￠n Guillermo、Zamarre?o Angel M.、Garc¨aa-Mina Jose M.、Rey-Stolle Valcarce Ma Fernanda、Barbas Coral、Franco-Zorrilla Jos¨| Manuel、Solano Roberto、Kneeshaw Sophie、Soriano Gonzalo
Department of Plant Molecular Genetics, Centro Nacional de Biotecnolog¨aa, Consejo Superior de Investigaciones Cient¨aficas (CNB-CSIC)Department of Environmental Biology, University of NavarraDepartment of Environmental Biology, University of NavarraCentre for Metabolomics and Bioanalysis (CEMBIO), Chemistry and Biochemistry Department, Pharmacy Faculty, Universidad San Pablo-CEUCentre for Metabolomics and Bioanalysis (CEMBIO), Chemistry and Biochemistry Department, Pharmacy Faculty, Universidad San Pablo-CEUDepartment of Plant Molecular Genetics, Centro Nacional de Biotecnolog¨aa, Consejo Superior de Investigaciones Cient¨aficas (CNB-CSIC)Department of Plant Molecular Genetics, Centro Nacional de Biotecnolog¨aa, Consejo Superior de Investigaciones Cient¨aficas (CNB-CSIC)Department of Plant Molecular Genetics, Centro Nacional de Biotecnolog¨aa, Consejo Superior de Investigaciones Cient¨aficas (CNB-CSIC)Department of Plant Molecular Genetics, Centro Nacional de Biotecnolog¨aa, Consejo Superior de Investigaciones Cient¨aficas (CNB-CSIC)||Facultad de Ciencia y Tecnolog¨aa, Universidad de La Rioja
生物化学分子生物学植物学
Jasmonateshexadecatrienoic acidfatty acid desaturaseMarchantia polymorphadn-OPDAphytohormones
Jimenez-Alem¨￠n Guillermo,Zamarre?o Angel M.,Garc¨aa-Mina Jose M.,Rey-Stolle Valcarce Ma Fernanda,Barbas Coral,Franco-Zorrilla Jos¨| Manuel,Solano Roberto,Kneeshaw Sophie,Soriano Gonzalo.An evolutionarily ancient Fatty Acid Desaturase is required for the synthesis of hexadecatrienoic acid, which is the main source of the bioactive jasmonate in Marchantia polymorpha[EB/OL].(2025-03-28)[2025-05-28].https://www.biorxiv.org/content/10.1101/2021.09.06.459162.点此复制
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