PCR-DGGE技术及其在植物微生态研究中的应用

he microorganism is very abundant in plant and plays importane roles in plant micro-ecology. As a fast and reliable molecular method，polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique has been proven to be a powerful tool for analyzing the microbial diversity and microbial communities and their dynamics changes in plant micro-ecology. In this paper,the principle and application aspects in plant micro-ecology, as well as the main causing factors in the limitation of DGGE techniques for microbial ecology analysis and the solutions were introduced. And it is expected to provide the basis for the analysis of the DGGE result and in the plant-microbial interaction research.