2,2'-联咪唑钌配合物与G-四链体DNA的作用研究
Studies of Ruthenium(Ⅱ)-2,2'-biiminazole complexes on binding to G-quadruplex DNA
本文合成并表征了三个钌(II)配合物[Ru(bpy)2(biim)]2+ (1)、[Ru(phen)2(biim)]2+ (2) 和[Ru(p-mopip)2(biim)]2+ (3)（bpy是联吡啶，phen是邻菲罗啉，p-mopip是4-甲氧基苯咪唑[4,5-f][1,10]菲罗啉）。采用圆二色谱（CD）、荧光共振能量转移（FRET）和PCR-stop实验方法研究了钌(II)配合物与人端粒DNA序列 5′-G3(T2AG3)3-3′(HTG21)的相互作用。结果表明配合物1、2能诱导人端粒DNA构象发生明显的改变，且三个配合物都能稳定G-四链体结构。
hree ruthenium(II) complexes [Ru(bpy)2(biim)]2+ (1), [Ru(phen)2(biim)]2+ (2) and [Ru(p-mopip)2(biim)]2+ (3) where bpy is 2,2′-bipyridine, phen is 1,10-phenanthroline, biim is 2,2′-biimidazole and p-mopip is 2-(4-methoxyphenyl)-imidazo- [4,5f]phenanthroline), have been synthesized and characterized in this work. The interaction of human telomeric DNA sequence 5′-G3(T2AG3)3-3′(HTG21) with ruthenium(II) complexes was investigated via circular dichroism (CD) measurements, fluorescence resonance energy transfer (FRET) melting assay, polymerase chain reaction (PCR) stop assay. The results indicated that complexes 1, 2 could induce a remarkable conformational change of human telomeric DNA, and three complexes have the ability to stabilize the G-quadruplex.
刘杰、夏钰
生物化学生物物理学分子生物学
配合物端粒DNAG-四链体
complexestelomeric DNAG-quadruplex
刘杰,夏钰.2,2'-联咪唑钌配合物与G-四链体DNA的作用研究[EB/OL].(2012-05-04)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/201205-51.点此复制
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